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IL-22 (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Interleukin-22

Correlated keywords:

ELISA

Chemokin

Th22

epithelial cells

keratinocytes

hepatocytes

cells

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 22 (IL-22) is one of the 9 members of the IL-10 family(1) (IL-10/IL-19/IL-20/IL-22/IL-24/IL-26/IL-28A/IL-28B and IL-29).
IL-22 is a polypeptide of 146 amino acids with a molecular weight of 16.7 kDa(2). IL-22 structure contains six antiparallel helices(3). It is produced at a monomeric state(4) by cells from the lymphoid lineage(5) (CD4+ T cells, Th22 subpopulation, NK cells) . This cytokine acts only on non-hematopoietic stromal cells, in particular epithelial cells, keratinocytes, and hepatocytes(6).
IL-22 increases the innate immunity of tissue cells (induction of antimicrobial molecules), protects tissues from damage, and enhances their regenerations(3).
Upregulation is find in many diseases like psoriasis and Crohn's disease.

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,02 ng/mL

Standard Curve Range: 0,02-2,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

1. Sabat R IL-10 family of cytokines. 2010, Cytokine Growth Factor Rev 21:315–324

2. Xie MH, Aggarwal S, Ho WH et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2–4 and IL-22R. 2000, J Biol Chem 275:31335–31339

3. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview. 2013, IL-17, IL-22 and Their Producing Cells: Role in Inflammation and Autoimmunity, 2nd ed., pp.11-35

4. Zdanov A Structural analysis of cytokines comprising the IL-10 family. 2010, Cytokine Growth Factor Rev 21:325–330

5. Trifari S, Kaplan CD, Tran EH et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells. 2009, Nat Immunol 10:864–871

6. Rutz S, Eidenschenk C, Ouyang W IL-22, not simply a Th17 cytokine. 2013, Immunol Rev. Mar;252(1):116-32

주문정보

CAT.No	품명	규격	비고
A05424	IL-22 (pig) ELISA kit	96 wells	HRP kits