Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
▶Ensures reliable calculation of bisulfite conversion efficiency post library prep.
▶Six unique amplicons with 0, 10, 25, 50, 75, and 100% methylation levels allow for a standard curve and robust data normalization.
▶Compatible with various species (except for E. coli) and bisulfite sequencing library preparation methods.
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
HIGHLIGHTS
Ensures reliable calculation of bisulfite conversion efficiency post library prep.
Six unique amplicons with 0, 10, 25, 50, 75, and 100% methylation levels allow for a standard curve and robust data normalization.
Compatible with various species (except for E. coli) and bisulfite sequencing library preparation methods.
DESCRIPTION
Zymo-Seq Methyl Spike-in Control is comprised of six unique double-stranded synthetic amplicons (180-200 bp) with distinct sequences derived from the E. coli K12 genome, and each amplicon represents a different CpG methylation level ranging from 0% to 100%. This control serves as an in situ control for next generation sequencing library preparations, providing an unbiased way of calculating the efficiency of the bisulfite conversion reaction. It can also be used to validate bioinformatics pipeline calibration by demonstrating a strong correlation between observed and expected methylation levels of the amplicons.
Concentration
60 pg/µl
Methylation Levels
Amplicon #
Methylation Level
1
0%
2
10%
3
25%
4
50%
5
75%
6
100%
Source
Double-stranded synthetic amplicons derived from the E. coli genome.
We recommend using 60pg of Zymo-Seq Methyl Spike-in Control for 100-500ng of sample DNA, 30pg for 50-99ng of DNA, and 10pg for 20-49ng of DNA. Depending on the required amount of the Zymo-Seq Methyl Spike-in Control per DNA sample, it can be further diluted to the appropriate concentration in TE buffer.
