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Quick-RNA Midiprep Kit
Highlights
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
High Yield: Recover up to 1 mg of RNA
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • High Yield: Recover up to 1 mg of RNA
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.

Product Description


The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.

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Technical Specifications

EquipmentMicrocentrifuge, vortex, vacuum manifold
PurityRNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample SourceCells or tissue samples, yeast, plant or bacteria.
Size RangeTotal RNA ≥ 17 nt
YieldMidiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)

Resources


Q1: Is DNase I available for individual purchase?

Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.

Q2: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q3: I ran out of RNA Wash Buffer. Can I use something else?

Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Q4: Will the kit isolate small RNAs?

Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

주문정보

CAT.No 품명 규격 비고
R1056 Quick-RNA Midiprep Kit 25 preps