"The Beauty of Science is to Make Things Simple"
Whether it's epigenetics, DNA, RNA, E. coli, or yeast based research, Zymo philosophy remains the same
: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.
HIGHLIGHTS
Simple: Quickly and easily rescue plasmid from yeast.
Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
Detail Description
Highlights
Simple: Quickly and easily rescue plasmid from yeast.
Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Product Description
The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
Technical Specifications
Applicable For
Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume
≥ 10 µl per well
Equipment
Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
Processing Volume
≤ 1.5 ml of Culture
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
Up to 25 kb.
Yield
Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Any strains susceptible to Zymolyase, which includes the following fungal genera: AsbyaKloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
