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Zymo Research
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ZR Plasmid Miniprep - Classic
Highlights

High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.

Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.

Unique column design: Zero buffer retention and low (30 µl) elution volume.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
  • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
  • Unique column design: Zero buffer retention and low (30 µl) elution volume.

 

Product Description

The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

Technical Specifications

Applicable ForLigation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.
Elution Volume≥ 30 µl
Endotoxin Levels< 50 EU/µg
EquipmentMicrocentrifuge
Processing Time15 min
PurityTypical Abs260/280 ≥1.8.
Size RangeUp to 25 kb
YieldUp to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.

Resources

Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?

The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.

Q2: Can the kit be used to isolate large constructs (BAC/PAC)?

No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.

Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?

Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

Q4: What is the composition of the DNA Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Q5: Can I perform the procedure using a vacuum manifold?

Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.

Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.

Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?

≤ 50 Endotoxin Units/µg of plasmid DNA.

Q8: I accidently left my P3 Buffer at room temperature, is it still okay to use?

Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

주문정보

CAT.No 품명 규격 비고
D4015 ZR Plasmid Miniprep - Classic 100 preps. Format: Spin-Column Sample Volume: 0.5 - 5.0 ml Processing Time: 15 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb
D4016 ZR Plasmid Miniprep - Classic 400 preps. Format: Spin-Column Sample Volume: 0.5 - 5.0 ml Processing Time: 15 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb
D4054 ZR Plasmid Miniprep - Classic 800 preps. Format: Spin-Column Sample Volume: 0.5 - 5.0 ml Processing Time: 15 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb