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Zymo Research
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ZR-96 DNA Clean-Up Kit
Highlights

Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.

ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.

Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
  • ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.



Product Description

The ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.

Technical Specifications

Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume≥ 30 µl for shallow well, ≥ 10 µl for deep well
EquipmentCentrifuge with microplate carriers
PurityA260/A280 > 1.8
Sample SourceDNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well
Yield≤ 5 µg total DNA can be recovered. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Resources

Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How to process naked DNA stored in DNA/RNA Shield?

Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

We recommend no more than 5 times as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.

 

주문정보

CAT.No 품명 규격 비고
D4017 ZR-96 DNA Clean-Up Kit (Shallow Well) 2 x 96 preps. Format: 96-Well, Shallow Well Elution Volume: ≥ 30 μl Processing Time: 20 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb
D4018 ZR-96 DNA Clean-Up Kit (Shallow Well) 4 x 96 preps. Format: 96-Well, Shallow Well Elution Volume: ≥ 30 μl Processing Time: 20 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb