Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Quick, high-throughput (96-well) recovery of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
Unique spin column for low volume elution of ultra-pure, high-yield DNA
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quick, high-throughput (96-well) recovery of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
Unique spin column for low volume elution of ultra-pure, high-yield DNA
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Product Description
The ZR-96 Genomic DNA Clean & Concentrator (DCC) is a 96-well gDNA clean up kit that provides quick (~20 minutes) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This 96-well gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Technical Specifications
Applicable For
Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume
≥ 15 µl of DNA Elution Buffer
Equipment
Centrifuge w/ microplate carriers
Purity
A260/A280 > 1.8, A260/A230 > 1.8
Sample Source
Enzymatic reactions or impure preparations containing genomic DNA
Sample storage
Eluted DNA can be used immediately or stored at ≤ -20°C
Size Range
> 50 bp and up to 200 kb
Yield
Up to 5 µg DNA. Recovery of DNA ranges from 70 - 95%
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
