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Universal cDNA Reverse Transcribed by Oligo dT Primer: Rat Normal Tissues

Species: Rat
Applications: NGS, qPCR
BioChains' Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rat whole bodies without fur. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning rat genes.

02)576-6303
info@kyongshin.co.kr

제품소개

BioChain's universal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65ºC for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1 µ l cDNA is good enough for one PCR reaction.

주문정보

CAT.No	품명	규격	비고
C4434567	Universal cDNA Reverse Transcribed by Oligo dT Primer: Rat Normal Tissues	100 rxn